General Cell Based Assays
Enzo Life Sciences Matrix metalloproteinase-12 (MMP-12) fluorometric drug discovery kit, GREEN (1x96wells)
The MMP-12 Fluorometric (also known as fluorimetric) Drug Discovery Kit, GREEN is a complete assay system designed to screen MMP-12 inhibitors using a quenched fluorogenic OMNIMMP Green substrate: 5-FAM-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys(TQ2W)-NH2 [5-FAM = 5-carboxyfluorescein; Nva=norvaline; TQ2W=quencher]. FAM fluorescence is thoroughly quenched by the TQ2W group until cleavage by MMPs separates the two moieties. The assays are performed in a convenient 96-well microplate format. The kit is useful to screen inhibitors of MMP-12, a potential therapeutic target. The compound NNGH is also included as a prototypic control inhibitor. Alternative name: Metalloelastase, Macrophage elastase. Applications: Fluorescent detection, HTS Activity assay.
ASSAYQUANT TECHNOLOGIES INC Protein 1000 kin assay AQT0872
PhosphoSens-Kinetic Kinase Activity Kit 1000 assays - AQT0872
STEMCELL Technologies Phalloidin iFluorTM 647
Fluorescent dye conjugates for detection of F-actin in cell culture experiments - 300 Tests
Thermo Scientific Production Chemicals and Services DOE AND INGALLS
NC3906641 TRIS USP GMP HIGH ASSAY
Cayman Chemical 12-Lipoxygenase leukocyte-ty
Immunogen: Full length recombinant mouse 12-lipoxygenase (leukocyte-type) • Host: Rabbit • Species Reactivity: (+) Mouse, porcine • Applications: ELISA, WB • MW = ~73 kDa
Meso Scale Discovery R-PLEX Rat IL-6 ASY
The Meso Scale Discovery R-PLEX Rat IL-6 Assay is a multiplex immunoassay panel that allows simultaneous quantitative measurement of rat interleukin 6 (IL-6) in serum plasma and cell culture supernatants This assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision with low sample volume requirements The assay requires 50 L or less of sample and has a testing time of 3 5 hours The lower limit of detection for rat IL-6 is 1 3 pg/mL This assay is for research use only and is not intended for diagnostic procedures It is available as a singleplex assay or as part of a multiplex panel
Eurofins Discoverx cAMP Hunter eXpress mGLP1R U2OS GPCR Assay, 2-Plate Kit. (95-0179E3CP5M)
The cAMP Hunter mGLP1R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. exendin-4) based activation of mGLP1R GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type mGLP1R, a naturally Gs coupled GPCR. Upon activation of mGLP1R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human NCAM-1 ASY
The R-PLEX Human NCAM-1 Assay Kit from Meso Scale Discovery is a multiplex immunoassay that measures NCAM-1 (Neural Cell Adhesion Molecule 1) in human serum plasma and cell culture supernatants This assay kit utilizes MSD s electrochemiluminescent detection technology and provides high sensitivity up to 5-logs of dynamic range and low sample volume requirements The kit includes a 96-well plate pre-coated with capture antibody detection antibody Read Buffer Calibrator Blend and Sample Diluent The assay has a typical lower limit of detection of 0 05 ng/mL and an upper limit of 500 ng/mL for NCAM-1
Eurofins Discoverx cAMP Hunter eXpress ADRB3 DLD1 GPCR Assay, 10-Plate Kit. (95-0182E13CP25L)
The cAMP Hunter ADRB3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. isoproterenol) based activation of ADRB3 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type ADRB3, a naturally Gs coupled GPCR. Upon activation of ADRB3, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human IRS-1 ASY
The R-PLEX Human IRS-1 Assay Kit from Meso Scale Discovery is a multiplex immunoassay that measures human IRS-1 in cell and tissue lysates It utilizes electrochemiluminescence detection technology and provides high sensitivity broad dynamic range and requires low sample volume The assay has high specificity and low cross-reactivity It includes a 96-well plate coated with capture antibody detection antibody read buffer wash buffer blocker reagents and calibrator The kit can test up to 80 samples in duplicate and has a testing time of 2 5 hours It is for research use only
Cayman Chemical EstradIol ELISA KIt 480Wel
Cayman’s Estradiol ELISA Kit is a competitive assay that can be used for the quantification of estradiol in plasma and serum. This assay has a range of 0.61-10,000 pg/ml with a sensitivity of approximately 20 pg/ml (80% B/B0)
ASSAYQUANT TECHNOLOGIES INC Protein 1000 kin assay AQT0449
PhosphoSens-Kinetic Kinase Activity Kit 1000 assays - AQT0449
ASSAYQUANT TECHNOLOGIES INC Protein 1000 kin assay AQT0897
PhosphoSens-Kinetic Kinase Activity Kit 1000 assays - AQT0897
ILEX LIFE SCIENCES LLC QuickZyme Human MMP9 Activity Assay Kit 96 well plate format 1/EA
QuickZyme Human MMP-9 Activity Assay Kit 96 Assays 96-well plate format immunoassay for active or total MMP-9 The QuickZyme Human MMP-9 Activity Assay is an enzyme activity immunoassay that enables you to specifically measure endogenous active MMP-9 OR total active MMP-9 (following activation of pro-MMP-9 on the plate with APMA) It can be used for the measurement of MMP-9 activity in various biological samples such as conditioned cell culture medium serum plasma urine synovial fluid and tissue homogenates This 96-well plate format assay is based on the QuickZyme technology using a modified pro-enzyme as a substrate which upon activation is able to release color from a chromogenic peptide substrate This multiplication step provides a unique assay sensitivity Assay Range 0 5 - 16 ng/ml (1 h incubation) 0 01-1 ng/ml (4 h incubation) Sensitivity 0 005 ng/ml Synonyms Matrix metalloproteinase-9 MMP9 92 kDa gelatinase 92 kDa type IV collagenase Gelatinase B GELB